Everything You Need to Know About Mesophilic Cheese Culture
What is Mesophilic Cheese Culture?
Mesophilic cheese culture is a type of bacterial culture used in cheese making that thrives at moderate temperatures, typically between 68-102°F (20-39°C). These cultures play a crucial role in the cheese making process, contributing to flavor development, texture, and the overall character of many popular cheese varieties.
Understanding the Basics of Mesophilic Culture
Mesophilic culture is primarily composed of lactic acid bacteria. These beneficial bacteria work by converting lactose (milk sugar) into lactic acid. This process is essential for:
- Lowering the pH of the milk
- Helping to form the curd
- Developing flavor compounds
- Preserving the cheese
Common bacteria in mesophilic cultures include Lactococcus lactis subsp. lactis and Lactococcus lactis subsp. cremoris.

How is Mesophilic Culture Used in Cheese Making?
Mesophilic culture is used in the cheese making process in several ways:
- It’s added to heated milk to begin the fermentation process
- The culture works to acidify the milk, preparing it for curd formation
- It continues to work during the aging process, developing flavors over time
- In some cheeses, it contributes to the formation of eyes or holes
The amount of culture used and the specific strains can greatly influence the final cheese product.
Benefits of Using Mesophilic Starter Culture
Mesophilic starter culture offers several benefits in cheese making:
- Versatility: It’s used in a wide variety of cheese types
- Flavor development: Contributes to complex flavor profiles
- Texture: Helps achieve desired textures in many cheeses
- Temperature flexibility: Works well at room temperature
- Preservation: Aids in natural cheese preservation

Types of Cheeses Made with Mesophilic Culture
Mesophilic culture is used in making a variety of cheeses, including:
- Cheddar
- Gouda
- Colby
- Monterey Jack
- Feta
- Camembert
- Brie
- Cottage cheese
- Cream cheese
- Chevre (goat cheese)
These cheeses range from soft to hard, fresh to aged, showcasing the versatility of mesophilic cultures.
Exploring Different Cheese Varieties Using Mesophilic Culture
Let’s explore how mesophilic culture contributes to some popular cheese varieties:

Cheddar
Mesophilic culture is crucial in developing Cheddar’s sharp, tangy flavor and firm texture. The culture continues to work during the aging process, contributing to the cheese’s complex flavor profile.
Brie and Camembert
In these soft-ripened cheeses, mesophilic culture works alongside surface molds to create their characteristic creamy texture and earthy flavors.
Feta
Mesophilic culture helps develop Feta’s tangy flavor and crumbly texture. It’s often used in combination with thermophilic culture for this cheese.
Common Cheeses Made with Mesophilic Starter Culture
Here’s a closer look at some common cheeses that use mesophilic starter culture:
Cheese Type | Texture | Flavor Profile |
---|---|---|
Cheddar | Firm | Sharp, tangy |
Gouda | Semi-hard to hard | Sweet, nutty |
Colby | Semi-hard | Mild, sweet |
Cream Cheese | Soft, spreadable | Mild, tangy |
Feta | Crumbly | Salty, tangy |
Creating Mesophilic Cultured Cheese at Home
Making cheese at home using mesophilic culture can be a rewarding experience. Here are some tips:
- Start with simple cheeses like cream cheese or feta
- Ensure your equipment is properly sterilized
- Use fresh, high-quality milk
- Follow recipes closely, especially regarding temperature and timing
- Be patient – cheese making takes time and practice
Choosing the Right Mesophilic Starter Culture
When selecting a mesophilic starter culture, consider these factors:
- The type of cheese you’re making
- Desired flavor profile
- Texture goals
- Single strain vs. mixed strain cultures
- Direct set vs. bulk starter options
Remember, different mesophilic cultures can produce varying results, even in the same type of cheese.
Factors to Consider When Selecting Mesophilic Culture
When choosing a mesophilic culture for your cheese making, keep these points in mind:
- Cheese recipe requirements
- Culture strength and activity level
- Storage capabilities
- Ease of use
- Consistency of results
- Cost-effectiveness

Your choice can significantly impact the success of your cheese making endeavors.
Comparing Mesophilic vs. Thermophilic Starter Cultures
While both are essential in cheese making, mesophilic and thermophilic cultures have distinct characteristics:
Characteristic | Mesophilic Culture | Thermophilic Culture |
---|---|---|
Optimal Temperature | 68-102°F (20-39°C) | 108-128°F (42-53°C) |
Common Cheese Types | Cheddar, Gouda, Brie | Mozzarella, Parmesan, Swiss |
Flavor Profile | Mild to sharp | Often more complex |
Acidification Rate | Generally slower | Typically faster |
Tips for Using Mesophilic Culture in Cheese Making
To get the best results when using mesophilic culture in your cheese making, consider these tips:
- Always use fresh culture for optimal activity
- Follow temperature guidelines closely to ensure proper culture activation
- Allow sufficient time for the culture to work before adding rennet
- Monitor pH levels throughout the cheese making process
- Experiment with different strains to find your preferred flavor profile
- Keep detailed records of your cheese making process for consistency
Benefits of Mesophilic Culture for Homemade Cheeses
Using mesophilic culture in home cheese making offers several advantages:
- Works well at room temperature, making it ideal for home environments
- Versatile, allowing you to make a wide variety of cheeses
- Generally easier to work with compared to thermophilic cultures
- Produces consistent results with proper handling
- Can be used to make both fresh and aged cheeses
- Contributes to natural preservation of the cheese

How to Make Mesophilic Cheese Culture at Home
While commercial cultures are readily available, some cheese makers prefer to make their own mesophilic cheese culture. Here’s a basic overview of the process:
- Start with raw milk or cultured buttermilk as a base
- Warm the milk to about 86°F (30°C)
- Allow the milk to ferment at room temperature for 12-24 hours
- Once thickened, refrigerate the culture
- Use within a week for best results
Note that homemade cultures may not be as consistent as commercial options and carry a higher risk of contamination.
Step-by-Step Guide to Creating Mesophilic Starter Culture
For those interested in making their own mesophilic starter culture, here’s a more detailed guide:
- Choose your milk base (raw milk or cultured buttermilk)
- Sterilize all equipment thoroughly
- Warm the milk to 86°F (30°C)
- Pour the milk into a sterilized glass jar
- Cover with a clean cloth or coffee filter
- Let sit at room temperature for 12-24 hours
- Check for thickening and a slightly sour smell
- Once set, refrigerate immediately
- Use within 5-7 days for best results
Common Mistakes to Avoid When Making Mesophilic Starter
When creating your own mesophilic starter culture, be aware of these common pitfalls:
- Using non-sterile equipment, leading to contamination
- Incorrect incubation temperature
- Overfermentation, resulting in an overly acidic starter
- Using ultra-pasteurized milk, which may not culture properly
- Failing to refrigerate the culture once it’s set
- Using the culture when it’s too old or shows signs of contamination
FAQs About Mesophilic Cheese Culture
Here are some frequently asked questions about mesophilic cheese culture:
Q: Can mesophilic culture be used for non-dairy cheeses?
A: While traditionally used for dairy cheeses, some cheese makers have experimented with using mesophilic cultures in non-dairy cheese alternatives. However, results may vary, and specialized cultures for non-dairy cheese making are often more suitable.
Q: How long does mesophilic starter culture last?
A: Commercial freeze-dried mesophilic cultures can last up to two years when stored properly in the freezer. Homemade liquid cultures typically last about a week when refrigerated.
Q: Can I use yogurt as a mesophilic starter culture?
A: While yogurt contains lactic acid bacteria, it’s typically made with thermophilic cultures. For mesophilic cheeses, it’s best to use a proper mesophilic culture or cultured buttermilk.

Troubleshooting Mesophilic Cheese Culture Issues
Even experienced cheese makers can encounter issues with mesophilic cultures. Here are some common problems and solutions:
Dealing with Contamination in Mesophilic Culture
Signs of contamination include off-odors, unusual colors, or mold growth. If you suspect contamination:
- Discard the culture immediately
- Review your sanitation procedures
- Check the quality of your milk source
- Ensure proper storage temperatures
Reviving Weak Mesophilic Starter Cultures
If your culture seems weak or slow to activate:
- Check the expiration date of your culture
- Ensure you’re using the correct amount of culture
- Verify that your incubation temperature is correct
- Consider using a fresh culture or switching brands

Conclusion: Mastering Mesophilic Cheese Culture
Mesophilic cheese culture is a versatile and essential tool in the cheese maker’s arsenal. Whether you’re crafting a sharp cheddar, a creamy brie, or a tangy feta, understanding and mastering the use of mesophilic cultures can elevate your cheese making to new heights.
Remember, cheese making is both an art and a science. While mesophilic cultures provide the scientific foundation, your creativity and experimentation will help you develop unique and delicious cheeses. Don’t be afraid to try different combinations of cultures, adjust aging times, or even create your own cheese recipes.
As you continue your cheese making journey, keep detailed notes of your processes and results. This will help you refine your techniques and consistently produce high-quality cheeses. With practice, patience, and a good understanding of mesophilic cultures, you’ll be well on your way to becoming a skilled artisanal cheese maker.
Happy cheese making!
What is mesophilic cheese culture?
- Optimal growth temperature: 68-102°F (20-39°C)
- Used for many cheese varieties, including Cheddar, Gouda, and Brie
- Produces lactic acid slowly, allowing for complex flavor development
- Typically contains Lactococcus and Leuconostoc species
- Contributes to texture, aroma, and flavor of cheese
- Available in direct-set (ready-to-use) or bulk starter forms
How do you make mesophilic cheese cultures at home?
- Start with store-bought cultured buttermilk or commercial mesophilic culture
- Sterilize a glass jar and utensils
- Heat milk to 72°F (22°C)
- Add culture (1 tablespoon per cup of milk)
- Cover and let ferment at room temperature for 12-24 hours
- Once thickened, refrigerate
- Use a portion as starter for your next batch
What is the difference between mesophilic and thermophilic cultures?
- Temperature: Mesophilic (68-102°F), Thermophilic (102-140°F)
- Cheese types: Mesophilic (Cheddar, Gouda), Thermophilic (Mozzarella, Parmesan)
- Acid production: Mesophilic (slower), Thermophilic (faster)
- Flavor profile: Mesophilic (complex, buttery), Thermophilic (sharp, tangy)
- Texture: Mesophilic (varied), Thermophilic (often stretchy or granular)
- Common bacteria: Mesophilic (Lactococcus), Thermophilic (Streptococcus, Lactobacillus)
What mesophilic bacteria is used in cheese?
- Lactococcus lactis subsp. lactis
- Lactococcus lactis subsp. cremoris
- Leuconostoc mesenteroides subsp. cremoris
- Lactococcus lactis subsp. lactis biovar diacetylactis
- Lactobacillus casei (in some cases)
- Lactobacillus plantarum (in some cases)
Mesophilic culture
- Definition: Bacteria that thrive at moderate temperatures (68-102°F)
- Function: Convert lactose to lactic acid, develop flavor compounds
- Common uses: Cheddar, Gouda, Colby, Feta, Camembert, and many others
- Characteristics: Slow acid production, complex flavor development
- Types: Direct-set (ready-to-use) or mother culture (for propagation)
- Storage: Often freeze-dried for long-term stability
Mesophilic cheese culture examples
- Cheddar: Sharp, tangy flavor
- Gouda: Smooth, sweet taste
- Brie: Soft, creamy texture with a white rind
- Camembert: Similar to Brie, but with a stronger flavor
- Feta: Crumbly texture with a tangy, salty taste
- Colby: Mild, sweet flavor
- Havarti: Creamy with a slightly acidic taste
- Monterey Jack: Mild, slightly sweet flavor
MA11 cheese Culture
- Type: Mesophilic aromatic culture
- Composition: Typically includes Lactococcus lactis subsp. lactis, Lactococcus lactis subsp. cremoris, and Leuconostoc mesenteroides subsp. cremoris
- Characteristics: Produces diacetyl for buttery flavor and CO2 for small eye formation
- Common uses: Gouda, Edam, Havarti, and other semi-soft cheeses
- Flavor profile: Contributes to a buttery, slightly tangy taste
- Format: Usually available as a freeze-dried powder
Cheddar cheese culture
- Primary culture: Often a blend of Lactococcus lactis subsp. lactis and Lactococcus lactis subsp. cremoris
- Optional adjuncts: Lactobacillus helveticus or Streptococcus thermophilus for flavor enhancement
- Function: Converts lactose to lactic acid, develops flavor compounds
- Characteristics: Slow acid production allows for proper curd formation and aging
- Common blends: MA11, MA4001, or MM100
- Aging: Culture continues to work during the aging process, developing sharp flavors
Mesophilic culture substitute
- Buttermilk: Contains mesophilic bacteria, use 1/4 cup per gallon of milk
- Yogurt: Choose plain, active culture yogurt, use 1/4 cup per gallon of milk
- Kefir: Rich in various bacteria, use 1/4 cup per gallon of milk
- Sour cream: Contains mesophilic bacteria, use 1/4 cup per gallon of milk
- Citric acid + Rennet: For quick cheese like ricotta or mozzarella
- Commercial freeze-dried cultures: Different blends available for specific cheese types